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The Gerecht lab (https://gerechtlab.johnshopkins.edu/web) is relocating to Duke University, Department of Biomedical Engineering and is seeking an Associate in Research for an interdisciplinary research program studying the interactions between cells and their microenvironments in vascular differentiation and morphogenesis.

Reporting to the principal investigator (PI), the Associate in Research will establish and implement research protocols for complex and/or multiple research studies. Provide scientific, training, grants, and reporting support and other research responsibilities involving the research activities of the lab. As part of a study team, collaborate with trainees, develop protocols, and supervise study-related activities for one or more studies. Focus of the activates will be on cell and molecular biology aspects including genetic manipulation of stem cells.

Specific Duties & Responsibilities:

Experimental Support:

- Independently conduct laboratory-based experiments. These assays will mainly focus on gene editing of stem cells and their derivatives using CRISPR and other methods for generating stable cell lines. These will include but are not limited to: tissue and mammalian cell culture, cloning, gene expression analysis, immunohistochemistry, immunofluorescence microscopy, flow cytometry, ELISA, protein arrays.

- Teach assays to other laboratory personnel and collaborate with personnel in laboratory-based experiments and assays for the various research projects

- May also provide support for animal studies in the lab including animal handling and maintenance, preparation for surgeries, occasional measurements, etc

- Interpret data, write methods and protocols.

Scientific Support:

- Support and collaborate with trainees and personnel in planning studies, drafting/editing scientific content, including research protocols, specific sections of grants, manuscripts, commentaries, editorials, review articles and book chapters

- Engage in scientific discussions with collaborators and funding organizations Reporting:

- In collaboration with the PI, track activities, milestones, and deliverables

- Provide support and submit progress reports for research activities

- Along with the PI, monitors use of resources in accordance with project plans and budgets, assist control of resources, in a collaborative manner

Minimum Qualifications:

Master's degree in related discipline. Five years related experience, specifically with genetic manipulation of mammalian cells and cloning. Additional education may substitute for required experience, to the extent permitted by the Duke equivalency formula.

Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

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